Javascript must be enabled to continue!
Sister chromatid cohesion establishment during DNA replication termination
View through CrossRef
Abstract
The cohesin complex tethers sister chromatids together from the moment they are generated in S-phase until their separation in anaphase
1,2
. This fundamental phenomenon, called sister chromatid cohesion, underpins orderly chromosome segregation. The replisome complex coordinates cohesion establishment with replication of parental DNA
3
. Cohesion can be established by cohesin complexes bound to DNA before replication
4,5
, but how replisome interaction with pre-loaded cohesin complexes results in cohesion is not known. Prevailing models suggest cohesion is established by replisome passage through the cohesin ring or by transfer of cohesin behind the replication fork by replisome components
5
. Unexpectedly, by visualising single replication forks colliding with pre-loaded cohesin complexes, we find that cohesin is pushed by the replisome to where a converging replisome is met. Whilst the converging replisomes are removed during DNA replication termination, cohesin remains on nascent DNA. We demonstrate that these cohesin molecules tether the newly replicated sister DNAs together. Our results support a new model where sister chromatid cohesion is established during DNA replication termination, providing important insight into the molecular mechanism of cohesion establishment.
Title: Sister chromatid cohesion establishment during DNA replication termination
Description:
Abstract
The cohesin complex tethers sister chromatids together from the moment they are generated in S-phase until their separation in anaphase
1,2
.
This fundamental phenomenon, called sister chromatid cohesion, underpins orderly chromosome segregation.
The replisome complex coordinates cohesion establishment with replication of parental DNA
3
.
Cohesion can be established by cohesin complexes bound to DNA before replication
4,5
, but how replisome interaction with pre-loaded cohesin complexes results in cohesion is not known.
Prevailing models suggest cohesion is established by replisome passage through the cohesin ring or by transfer of cohesin behind the replication fork by replisome components
5
.
Unexpectedly, by visualising single replication forks colliding with pre-loaded cohesin complexes, we find that cohesin is pushed by the replisome to where a converging replisome is met.
Whilst the converging replisomes are removed during DNA replication termination, cohesin remains on nascent DNA.
We demonstrate that these cohesin molecules tether the newly replicated sister DNAs together.
Our results support a new model where sister chromatid cohesion is established during DNA replication termination, providing important insight into the molecular mechanism of cohesion establishment.
Related Results
The histone methyltransferase NSD3 contributes to sister chromatid cohesion and to cohesin loading at mitotic exit
The histone methyltransferase NSD3 contributes to sister chromatid cohesion and to cohesin loading at mitotic exit
Abstract
Sister chromatid cohesion guarantees the correct transmission of chromosomes to daughter cells, and this multi-step process occurs throughout the cell cycl...
Sister chromatid cohesion defects are associated with chromosome instability in Hodgkin lymphoma cells
Sister chromatid cohesion defects are associated with chromosome instability in Hodgkin lymphoma cells
Abstract
Background
Chromosome instability manifests as an abnormal chromosome complement and is a pathogenic event in cancer. Although a correla...
The histone methyltransferase NSD3 contributes to sister chromatid cohesion and to cohesin loading at mitotic exit
The histone methyltransferase NSD3 contributes to sister chromatid cohesion and to cohesin loading at mitotic exit
ABSTRACT
Sister chromatid cohesion is a multi-step process implemented throughout the cell cycle to ensure the correct transmission of chromosomes to daughter cel...
Genome wide hypomethylation and youth-associated DNA gap reduction promoting DNA damage and senescence-associated pathogenesis
Genome wide hypomethylation and youth-associated DNA gap reduction promoting DNA damage and senescence-associated pathogenesis
Abstract
Background: Age-associated epigenetic alteration is the underlying cause of DNA damage in aging cells. Two types of youth-associated DNA-protection epigenetic mark...
Genome wide hypomethylation and youth-associated DNA gap reduction promoting DNA damage and senescence-associated pathogenesis
Genome wide hypomethylation and youth-associated DNA gap reduction promoting DNA damage and senescence-associated pathogenesis
Introduction: The United States currently faces two opioid crises, an evolved crisis currently manifesting as widespread abuse of illicit opioids, and a crisis in pain management l...
Intrinsic RNA hairpin-mediated transcription termination at high temperature in
Thermus aquaticus
Intrinsic RNA hairpin-mediated transcription termination at high temperature in
Thermus aquaticus
ABSTRACT
Transcription termination establishes gene boundaries and limits regulatory interference. In bacteria, intrinsic termination, mediated b...
DNA damage in telophase leads to coalescence between segregated sister chromatid loci
DNA damage in telophase leads to coalescence between segregated sister chromatid loci
SummaryThe generation of DNA double strand breaks (DSBs) pose a high risk for the maintenance of the genome. Cells repair DSBs through two major mechanisms: non-homologous end join...
Fork coupling directs DNA replication elongation and termination
Fork coupling directs DNA replication elongation and termination
DNA replication is initiated at multiple loci to ensure timely duplication of eukaryotic genomes. Sister replication forks progress bidirectionally, and replication terminates when...

