Development, Validation, and Greenness Assessment of HPLC and ATR-FTIR for Mangiferin Quantitative Analysis in Raw Material

Due to the benefits of mangiferin, including antidiabetic, antibacterial, antiviral, antioxidant, and anti-inflammatory activities, it is a natural raw material used in botanical health and cosmetic products. This research aims to develop and validate analytical methods for the quantification of mangiferin in raw material, isolated from Mangifera indica L. leaves, using high performance liquid chromatography (HPLC) and attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR). The greenness assessment of both analytical methods was performed. The results indicated that both methods were validated in terms of specificity, linearity, range, detection limit, quantitation limit, accuracy, and precision. The ranges of mangiferin used in HPLC and ATR-FTIR were 30 to 360 µg/mL and 5.00 to 27.27% w/w, respectively. The mangiferin content in five batches, analyzed by using the validated HPLC and ATR-FTIR, ranged from 88.35 to 109.4% w/w and 90.7 to 111.8% w/w, respectively. Statistical analysis using a paired t-test revealed no significant difference in the mangiferin quantity between the two techniques at a 95% confidence level. The greenness scores of the HPLC and ATR-FTIR determined according to AGREE metric were 0.49 and 0.81, respectively. Both methods can be applied to quantify mangiferin content and control the quality of mangiferin raw material. Additionally, the validated ATR-FTIR, which proposed excellent environmental friendliness, was suggested as an alternative analytical method for routine assay of mangiferin raw material.