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Neutrophil Chemotaxis in rhesus macaques (Macaca mulatta)
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SUMMARY
Chemotactic responses of isolated peripheral blood neutrophils from rhesus macaques (Macaca mulatta) were studied, using a micropore filter method. Cell migration toward zymosan-activated serum was similar to that of human cells, whereas the response to N-formyl methionyl leucyl phenylalanine (fmlp) was weaker than was that in human cells, requiring higher concentrations of fmlp for maximal migration. Optimal fmlp concentrations for attraction of rhesus neutrophils and human neutrophils were 5.0 × 10−7M and 1.0 × 10−8M, respectively. The chemotactic responses of the 2 neutrophils to complement (zymosan-activated serum) were similar. However, rhesus neutrophils required a higher concentration of the formyl peptide, fmlp, for maximal migratory response.
American Veterinary Medical Association (AVMA)
Title: Neutrophil Chemotaxis in rhesus macaques (Macaca mulatta)
Description:
SUMMARY
Chemotactic responses of isolated peripheral blood neutrophils from rhesus macaques (Macaca mulatta) were studied, using a micropore filter method.
Cell migration toward zymosan-activated serum was similar to that of human cells, whereas the response to N-formyl methionyl leucyl phenylalanine (fmlp) was weaker than was that in human cells, requiring higher concentrations of fmlp for maximal migration.
Optimal fmlp concentrations for attraction of rhesus neutrophils and human neutrophils were 5.
0 × 10−7M and 1.
0 × 10−8M, respectively.
The chemotactic responses of the 2 neutrophils to complement (zymosan-activated serum) were similar.
However, rhesus neutrophils required a higher concentration of the formyl peptide, fmlp, for maximal migratory response.
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