Hydrophilic Interaction Liquid Chromatography

Abstract Chromatography is categorized after the type of mobile phase used, liquid, gas, or supercritical chromatography. Liquid chromatography is a very important separation method used in practically all chemistry fields. This chapter focuses on the basic theory necessary for a deeper understanding of the separation process. It covers new trends in liquid chromatography today. Analytical (linear) chromatography can be described by relatively simple models: injection of an n component mixture will give n Gaussian‐shaped peaks, which are more or less separated in the chromatogram. The chapter also focuses on analytical (linear) models assuming Gaussian peaks. It also talks about basic retention models and kinetics, band broadening and the plate height concept, sources of zone broadening, and dependence of zone broadening on flow rate. The new technique using small particles was given the name high‐performance liquid chromatography (HPLC).