Search engine for discovering works of Art, research articles, and books related to Art and Culture
Javascript must be enabled to continue!
Lysozyme Treatment Makes 16S rRNA Amplicon Sequencing Results Less Biased
View through CrossRef
Abstract
Background
Amplicon sequencing is widely applied in gut bacteria structure analysis. However, the proportion of Gram-positive bacteria may greatly affect the results of microbial community analysis. Lysozyme is an effective agent to extract DNA of Gram-positive bacteria. In this study, we assessed the influence of lysozyme treatment on results of Bactrocere dorsalis rectal bacteria structure.
Result
The results indicated that the total bacteria content can be significantly increased in lysozyme treated samples. Moreover, rectal bacteria diversity was significantly higher in lysozyme treated samples. A detail analysis revealed that abundance of Gram-positive bacteria significantly increased in samples treated with lysozyme.
Conclusion
This study indicates that lysozyme treatment before DNA extraction is an effective way to reduce bias in bacteria structure analysis, especially for samples with high proportion of Gram-positive bacteria.
Title: Lysozyme Treatment Makes 16S rRNA Amplicon Sequencing Results Less Biased
Description:
Abstract
Background
Amplicon sequencing is widely applied in gut bacteria structure analysis.
However, the proportion of Gram-positive bacteria may greatly affect the results of microbial community analysis.
Lysozyme is an effective agent to extract DNA of Gram-positive bacteria.
In this study, we assessed the influence of lysozyme treatment on results of Bactrocere dorsalis rectal bacteria structure.
Result
The results indicated that the total bacteria content can be significantly increased in lysozyme treated samples.
Moreover, rectal bacteria diversity was significantly higher in lysozyme treated samples.
A detail analysis revealed that abundance of Gram-positive bacteria significantly increased in samples treated with lysozyme.
Conclusion
This study indicates that lysozyme treatment before DNA extraction is an effective way to reduce bias in bacteria structure analysis, especially for samples with high proportion of Gram-positive bacteria.
Related Results
Pipeline for species-resolved full-length16S rRNA amplicon nanopore sequencing analysis of low-complexity bacterial microbiota
Pipeline for species-resolved full-length16S rRNA amplicon nanopore sequencing analysis of low-complexity bacterial microbiota
Abstract
16S rRNA amplicon sequencing is a fundamental tool for characterizing prokaryotic microbial communities. While short-read 16S rRNA sequencing is a proven s...
238. Direct identification of Bacterial Species with MinION Nanopore Sequencer In Clinical Specimens Suspected of Polybacterial Infection
238. Direct identification of Bacterial Species with MinION Nanopore Sequencer In Clinical Specimens Suspected of Polybacterial Infection
Abstract
Background
Conventional culture tests usually identify only a few bacterial species, which can grow well in the culture...
Identification of bacterial pathogens from clinical samples using 16S rRNA sequencing
Identification of bacterial pathogens from clinical samples using 16S rRNA sequencing
Introduction: Bacterial infections have a substantial impact on global health and can become serious if misdiagnosed with several diseases related to the central nervous, cardiovas...
Deep Learning for Predicting 16S rRNA Gene Copy Number
Deep Learning for Predicting 16S rRNA Gene Copy Number
ABSTRACTBackgroundCulture-independent 16S rRNA gene metabarcoding is a commonly used method in microbiome profiling. However, this approach can only reflect the proportion of seque...
Evaluation of 16S rRNA Gene Sequence for DNA Barcoding of Tuna Fish
Evaluation of 16S rRNA Gene Sequence for DNA Barcoding of Tuna Fish
For fish product authentication, DNA barcoding has been a reliable tool. This is due to its requirement of a small amount of tissue sample in order to conduct a full analysis for s...
Effect of Lysozyme on Mycobacteria
Effect of Lysozyme on Mycobacteria
AbstractThe effect of lysozyme on the growth of several strains of mycobacteria was examined at pH 5.0–7.0 in Dubos medium containing various concentrations of lysozyme (100–2,000 ...
Separation of lysozyme-ovotransferrin complexes and the cooperative role of their components in egg white
Separation of lysozyme-ovotransferrin complexes and the cooperative role of their components in egg white
Abstract
A complex of ovotransferrin and lysozyme was directly isolated from egg white using an anti-transferrin antibody-immobilized membrane after antiserum proteins were...
Phylogenetic analyses of the genus Glaciecola: emended description of the genus Glaciecola, transfer of Glaciecola mesophila, G. agarilytica, G. aquimarina, G. arctica, G. chathamensis, G. polaris and G. psychrophila to the genus Paraglaciecola gen. nov.
Phylogenetic analyses of the genus Glaciecola: emended description of the genus Glaciecola, transfer of Glaciecola mesophila, G. agarilytica, G. aquimarina, G. arctica, G. chathamensis, G. polaris and G. psychrophila to the genus Paraglaciecola gen. nov.
Phylogenetic analyses of the genusGlaciecolawere performed using the sequences of the 16S rRNA gene and the GyrB protein to establish its taxonomic status. The results indicated a ...