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Laboratory Protocols for Ancient and Modern Dental Calculus DNA Processing (Fellows Yates et al. 2021) v1
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Collection of protocols used for Fellows Yates et al. "The evolution and changing ecology of the hominid primate oral microbiome". Bioinformatics analysis can be found on GitHub at https://github.com/jfy133/Anthropoid_Calculus_Microbiome_Evolution/. This collection describes the laboratory procedures used for sampling, (ancient) DNA extraction, library construction and preparation for Illumina sequencing of ancient and modern dental calculus samples. Sampling - Dental calculus Field-Sampling Protocol (Warinner Version) - Dental Calculus Field-Sampling Protocol (Sabin version) Extraction - Ancient DNA Extraction from Dental Calculus with Consolidant Removal - DNA Extraction from Modern Dental Calculus Library preparation - Non-UDG treated double-stranded DNA library preparation for Illumina sequencing ofancient dental calculus - (Non-UDG treated) double-stranded modern dental calculus DNA library preparation for Illumina sequencing - Full-UDG treated double-stranded ancient DNA library preparation for Illumina sequencing of ancient dental calculus (reduced input DNA) Indexing - Illumina double-stranded DNA dual indexing for ancient DNA Preparation for Sequencing - Amplification and Pooling
Springer Science and Business Media LLC
Title: Laboratory Protocols for Ancient and Modern Dental Calculus DNA Processing (Fellows Yates et al. 2021) v1
Description:
Collection of protocols used for Fellows Yates et al.
"The evolution and changing ecology of the hominid primate oral microbiome".
Bioinformatics analysis can be found on GitHub at https://github.
com/jfy133/Anthropoid_Calculus_Microbiome_Evolution/.
This collection describes the laboratory procedures used for sampling, (ancient) DNA extraction, library construction and preparation for Illumina sequencing of ancient and modern dental calculus samples.
Sampling - Dental calculus Field-Sampling Protocol (Warinner Version) - Dental Calculus Field-Sampling Protocol (Sabin version) Extraction - Ancient DNA Extraction from Dental Calculus with Consolidant Removal - DNA Extraction from Modern Dental Calculus Library preparation - Non-UDG treated double-stranded DNA library preparation for Illumina sequencing ofancient dental calculus - (Non-UDG treated) double-stranded modern dental calculus DNA library preparation for Illumina sequencing - Full-UDG treated double-stranded ancient DNA library preparation for Illumina sequencing of ancient dental calculus (reduced input DNA) Indexing - Illumina double-stranded DNA dual indexing for ancient DNA Preparation for Sequencing - Amplification and Pooling.
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