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Molecular typing methods to characterize Brucella spp. from animals: A review
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Brucellosis is an infectious disease of animals that can infect humans. The disease causes significant economic losses and threatens human health. A timely and accurate disease diagnosis plays a vital role in the identification of brucellosis. In addition to traditional diagnostic methods, molecular methods allow diagnosis and typing of the causative agent of brucellosis. This review will discuss various methods, such as Bruce-lаdder, Suiladder, high-resolution melt analysis, restriction fragment length polymorphism, multilocus sequence typing, multilocus variable-number tandem repeat analysis, and whole-genome sequencing single-nucleotide polymorphism, for the molecular typing of Brucella and discuss their advantages and disadvantages.
Keywords: Brucella, molecular typing, multilocus sequence typing, multilocus variable-number tandem-repeat analysis, single-nucleotide polymorphisms, whole-genome sequencing.
Veterinary World
Title: Molecular typing methods to characterize Brucella spp. from animals: A review
Description:
Brucellosis is an infectious disease of animals that can infect humans.
The disease causes significant economic losses and threatens human health.
A timely and accurate disease diagnosis plays a vital role in the identification of brucellosis.
In addition to traditional diagnostic methods, molecular methods allow diagnosis and typing of the causative agent of brucellosis.
This review will discuss various methods, such as Bruce-lаdder, Suiladder, high-resolution melt analysis, restriction fragment length polymorphism, multilocus sequence typing, multilocus variable-number tandem repeat analysis, and whole-genome sequencing single-nucleotide polymorphism, for the molecular typing of Brucella and discuss their advantages and disadvantages.
Keywords: Brucella, molecular typing, multilocus sequence typing, multilocus variable-number tandem-repeat analysis, single-nucleotide polymorphisms, whole-genome sequencing.
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