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Sanger Tree of Life RNA Extraction: Manual TRIzol™ v1
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This protocol describes the manual extraction of RNA from multiple different tissue samples intended for RNA-Seq, using the TRIzol™ Reagent, based on the Thermo Fisher TRIzol™ Reagent User Guide. This process is highly effective for most taxonomic groups covered by the Tree of Life Programme. The output of this protocol is a highly concentrated RNA extract which can be diluted and submitted for both RNA-Seq and IsoSeq.
Springer Science and Business Media LLC
Title: Sanger Tree of Life RNA Extraction: Manual TRIzol™ v1
Description:
This protocol describes the manual extraction of RNA from multiple different tissue samples intended for RNA-Seq, using the TRIzol™ Reagent, based on the Thermo Fisher TRIzol™ Reagent User Guide.
This process is highly effective for most taxonomic groups covered by the Tree of Life Programme.
The output of this protocol is a highly concentrated RNA extract which can be diluted and submitted for both RNA-Seq and IsoSeq.
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