Search engine for discovering works of Art, research articles, and books related to Art and Culture
Javascript must be enabled to continue!
Multisite Gateway Calculations: Excel spreadsheet v1
View through CrossRef
Multisite Gateway cloning provides a modular tool to assemble plasmid vectors suitable for numerous applications. The assembly of Multisite Gateway plasmids requires the correct ratios of three entry vectors and the destination backbone to favor correct recombination by Gateway LR Clonase II Plus. While simple, the calculation of recombination mixes is a reoccurring task when using Multisite Gateway that benefits from automation and proper documentation. This protocol provides a simple Excel-based spreadsheet to simplify the involved calculations based on providing individual vector lengths and working stock concentrations. Using this Multisite Gateway calculations spreadsheet supports correct reaction mix assembly, scaling of cloning reactions, cloning documentation, and troubleshooting.
Title: Multisite Gateway Calculations: Excel spreadsheet v1
Description:
Multisite Gateway cloning provides a modular tool to assemble plasmid vectors suitable for numerous applications.
The assembly of Multisite Gateway plasmids requires the correct ratios of three entry vectors and the destination backbone to favor correct recombination by Gateway LR Clonase II Plus.
While simple, the calculation of recombination mixes is a reoccurring task when using Multisite Gateway that benefits from automation and proper documentation.
This protocol provides a simple Excel-based spreadsheet to simplify the involved calculations based on providing individual vector lengths and working stock concentrations.
Using this Multisite Gateway calculations spreadsheet supports correct reaction mix assembly, scaling of cloning reactions, cloning documentation, and troubleshooting.
Related Results
Centaurs transitioning to JFCs: thermal and dynamical evolution
Centaurs transitioning to JFCs: thermal and dynamical evolution
<p>1- Context</p>
<p>Jupiter-family Comets are continuously replenished from their outer solar system reservoirs. Before they enter the in...
Prediction of multisite pain incidence in adolescence using a machine learning approach
Prediction of multisite pain incidence in adolescence using a machine learning approach
Abstract
Importance
Multisite pain is a major adverse health outcome in the adolescent population, affecting the daily lives of...
Symmetry breaking meets multisite modification
Symmetry breaking meets multisite modification
Multisite modification is a basic way of conferring functionality to proteins and a key component of post-translational modification networks. Additional interest in multisite modi...
Penggunaan SMS Gateway pada Sistem Informasi Pengasuhan dan Pengajaran Pesantren Berbasis Web
Penggunaan SMS Gateway pada Sistem Informasi Pengasuhan dan Pengajaran Pesantren Berbasis Web
AbstractNowadays information systems are widely chosen as media to help human activities in a way that is more efficient both in energy and in time. The registry of modern boarding...
Spreadsheet Implementation Technology
Spreadsheet Implementation Technology
A guide to innovative spreadsheet implementation technology, accompanied by a free software platform for experimentation.
Spreadsheets are used daily by millions of ...
Multi-Gateway-Based Energy Holes Avoidance Routing Protocol for WSN
Multi-Gateway-Based Energy Holes Avoidance Routing Protocol for WSN
In wireless sensor networks (WSNs), efficient energy conservation is required to prolong the lifetime of the network. In this work, we have given emphasis on balanced energy consum...
PELATIHAN MICROSOFT EXCEL TINGKAT LANJUT KARYAWAN PT. NUTRISI JUARA ASIA
PELATIHAN MICROSOFT EXCEL TINGKAT LANJUT KARYAWAN PT. NUTRISI JUARA ASIA
Microsoft Excel adalah program spreadsheet yang memungkinkan pengguna untuk membuat, mengelola, dan menganalisis data dalam format tabel atau lembar kerja. Excel berguna untuk mela...
Layout inference and table detection in spreadsheet document
Layout inference and table detection in spreadsheet document
Spreadsheet applications have evolved to be a tool of great importance for businesses,
open data, and scientific communities. Using these applications, users can perform various t...