Retraction

Retraction: Francis Domain, Xiaowen R. Bina and Stuart B. Levy, “Transketolase A, an enzyme in central metabolism, derepresses the marRAB multiple antibiotic resistance operon of Escherichia coli by interaction with MarR”, Molecular Microbiology 2007, 66(2), 383‐393 ( https://doi/10.1111/j.1365-2958.2007.05928.x ). The above article, published online on 14 September 2007 in Wiley Online Library ( wileyonlinelibrary.com ), has been retracted by agreement between the authors, the journal Editor in Chief John Helmann, and John Wiley & Sons Ltd. The retraction has been agreed because additional work in the authors' laboratory (L.M. McMurry) failed to confirm that the tktA gene overexpressed from pSPOK–TktA enhanced the expression in vivo of the mar promoter fusion marO–lacZ , or that deletion of chromosomal tktA reduced marO–lacZ expression. The original conclusions probably resulted from a yet unexplained variability of LacZ activity later discovered among different isolates of the marO–lacZ indicator strain SPC105. Moreover, the apparent binding of MarR to TktA‐6H immobilized on NiNTA resin (Fig. 1B) was an artefact caused by non‐specific binding of MarR alone to the resin that occurred when the wash contained no imidazole. Most MarR eluted in 20 mM imidazole without TktA‐6H. Therefore, the conclusion that TktA interacts physically with MarR is not warranted. It is noted that Xiaowen R. Bina was not involved in work related to these errors. Note that previously a corrigendum was published to note these concerns (Molecular Microbiology (2011) 80(3), 853).