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Effects of tea polyphenols and theaflavins on three oral caridogenic bacteria
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In order to investigate the antibacterial mechanism of tea polyphenols
(TPS) and theaflavins against oral cariogenic bacteria. The pH value of
the culture medium, the number of bacteria adhering to the smooth glass
tube wall, and the electrical conductivity value within 10 hours were
measured, respectively. The effects of four concentrations of tea
polyphenols and theaflavins below the MIC value were studied on acid
production, adhesion, and electrical conductivity of oral cariogenic
bacteria. The live/dead staining method was used to observe the effects
of four concentrations of TP and theaflavins below MIC value on the
biofilm activity of oral cariogenic bacteria under a laser scanning
confocal microscope.With the increasing concentrations of tea
polyphenols and theaflavins, the acid production and adhesion of the
cariogenic bacteria gradually decreased, and the conductivity gradually
increased. However, the conductivity increase was not significant
(P<0.05). Compared with the control group, the 1/2MIC and
1/4MIC TP and theaflavins treatments significantly reduced the biomass
of cariogenic biofilm (P<0.05). CLSM showed that the
integrated optical density of green fluorescence of the cariogenic
biofilm gradually decreased with the increase of drug concentration
after the action of tea polyphenols and theaflavins.
Title: Effects of tea polyphenols and theaflavins on three oral caridogenic bacteria
Description:
In order to investigate the antibacterial mechanism of tea polyphenols
(TPS) and theaflavins against oral cariogenic bacteria.
The pH value of
the culture medium, the number of bacteria adhering to the smooth glass
tube wall, and the electrical conductivity value within 10 hours were
measured, respectively.
The effects of four concentrations of tea
polyphenols and theaflavins below the MIC value were studied on acid
production, adhesion, and electrical conductivity of oral cariogenic
bacteria.
The live/dead staining method was used to observe the effects
of four concentrations of TP and theaflavins below MIC value on the
biofilm activity of oral cariogenic bacteria under a laser scanning
confocal microscope.
With the increasing concentrations of tea
polyphenols and theaflavins, the acid production and adhesion of the
cariogenic bacteria gradually decreased, and the conductivity gradually
increased.
However, the conductivity increase was not significant
(P<0.
05).
Compared with the control group, the 1/2MIC and
1/4MIC TP and theaflavins treatments significantly reduced the biomass
of cariogenic biofilm (P<0.
05).
CLSM showed that the
integrated optical density of green fluorescence of the cariogenic
biofilm gradually decreased with the increase of drug concentration
after the action of tea polyphenols and theaflavins.
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