The characteristic change of mRNA expression profile in plasmaexosomes from patients with MPO-ANCA associated vasculitis and itspossible connections with pathogenesis

Abstract Objective To explore the expression characteristics and potential roles of mRNAs in exosomes from the patients with MPO-AAV. Methods Plasma exosomes were isolated from MPO-AAV patients and HC to conducted exosome mRNA sequencing to screen differential mRNA expression in MPO-AAV compared to HC. The mRNA differentially expressed in exosomes from the 2 groups were comparatively explored by bioinformatics analysis. Six mRNAs with most differential expressions were selected and validated in larger groups of MPO-AAV patient and HC by RT-PCT. The relationships between these selected mRNAs and patient clinical information will be statistically analyzed. Results Compared with HCs, a total of 1,077 mRNA in exosomes from MPO-AAV patients were found to be differentially expressed mRNAs significantly up-regulated included DEPDC1B and TPST1, while NSUN4 and AK4 were involved in mRNAs significantly down regulated. Statistics analysis did not find any correlation-ship between the six selected mRNAs and the patient clinical indicators including disease activity. Conclusions There are significant differences in the expression characteristics of exosome mRNA between MPO-AAV patients and healthy people, which may be related to the occurrence and development of MPO-AAV, which provides the clues for further investigation of MPO-AAV pathogenesis and further search for new potential therapeutic targets.