Characterization of leucine zipper complexes by electrospray ionization mass spectrometry

AbstractThe development of “soft” ionization methods has enabled the mass spectrometric analysis of higher‐order structural features of proteins. We have applied electrospray ionization mass spectrometry (ESI‐MS) to the analysis of the number and composition of polypeptide chains in homomeric and heteromeric leucine zippers. In comparison with other methods that have been used to analyze leucine zippers, such as analytical ultracentrifugation, gel chromatography, or electrophoretic band shift assays, ESI‐MS is very fast and highly sensitive and provides a straightforward way to distinguish between homomeric and heteromeric coiled‐coil structures. ESI‐MS analyses were carried out on the parallel dimeric leucine zipper domain GCN4‐pl of the yeast transcription factor GCN4 and on three synthetic peptides with the sequences Ac‐EYEALEKKLAAX1EAKX2QALEKKLEALEHG‐amide: peptide LZ (X1, X2 = Leu), peptide LZ(12A) (X1 = Ala, X2 = Leu), and peptide LZ(16N) (X1 = Leu, X2 = Asn). Equilibrium ultracentrifugation analysis showed that LZ forms a trimeric coiled coil and this could be confirmed unequivocally by ESI‐MS as could the dimeric nature of GCN4‐pl. The formation of heteromeric two‐ and three‐stranded leucine zippers composed of chains from LZ and LZ(12A), or from GCN4‐pl and LZ, was demonstrated by ESI‐MS and confirmed by fluorescence quenching experiments on fluorescein‐labeled peptides. The results illustrate the adaptability and flexibility of the leucine zipper motif, properties that could be useful to the design of specific protein assemblies by way of coiled‐coil domains.