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IN VITRO HYPOGLYCEMIC AND ANTIMICROBIAL ACTIVITY OF CUCUMIS CALLOSUS (ROTTL.) COGN. FRUIT
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</xml><![endif]--><p>ABSTRACT<br />Objective: This research investigated in vitro hypoglycemic and antimicrobial activity of methanolic fruit extract of Cucumis callosus (MECC) (Rottl.)<br />cogn.<br />Methods: The in vitro antidiabetic ability of MECC was evaluated by inhibition studies of the digestive enzymes including α-amylase and α-glycosidase.<br />The antimicrobial activity of extract was further evaluated against 21 laboratory strains belongs to 8 bacterial species and 4 fungal species using cupplate<br />agar<br />diffusion assay<br />and agar<br />dilution assay.<br />Results: Our assay result suggested that the MECC exhibited dose-dependent increase in percentage inhibitory activity on α-glycosidase<br />(IC<br />50 <br />405.37 µg/ml) and α-amylase enzymes (IC<br />721.55 µg/ml). The results of antimicrobial activity also showed that the extract inhibited the growth<br />of 13 strains of 7 bacterial species and all fungal species.<br />50 <br />Conclusion: The observed inhibitions of α-amylase and α-glycosidase and inhibition of some microbial species suggested that the MECC may be useful<br />in the management of diabetes mellitus and treatment of some infectious diseases.<br />Keywords: Cucumis callosus, Diabetes, α-amylase, α-glycosidase, Antimicrobial.</p><!--[if gte mso 9]><xml>
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Innovare Academic Sciences Pvt Ltd
Title: IN VITRO HYPOGLYCEMIC AND ANTIMICROBIAL ACTIVITY OF CUCUMIS CALLOSUS (ROTTL.) COGN. FRUIT
Description:
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</xml><![endif]--><p>ABSTRACT<br />Objective: This research investigated in vitro hypoglycemic and antimicrobial activity of methanolic fruit extract of Cucumis callosus (MECC) (Rottl.
)<br />cogn.
<br />Methods: The in vitro antidiabetic ability of MECC was evaluated by inhibition studies of the digestive enzymes including α-amylase and α-glycosidase.
<br />The antimicrobial activity of extract was further evaluated against 21 laboratory strains belongs to 8 bacterial species and 4 fungal species using cupplate<br />agar<br />diffusion assay<br />and agar<br />dilution assay.
<br />Results: Our assay result suggested that the MECC exhibited dose-dependent increase in percentage inhibitory activity on α-glycosidase<br />(IC<br />50 <br />405.
37 µg/ml) and α-amylase enzymes (IC<br />721.
55 µg/ml).
The results of antimicrobial activity also showed that the extract inhibited the growth<br />of 13 strains of 7 bacterial species and all fungal species.
<br />50 <br />Conclusion: The observed inhibitions of α-amylase and α-glycosidase and inhibition of some microbial species suggested that the MECC may be useful<br />in the management of diabetes mellitus and treatment of some infectious diseases.
<br />Keywords: Cucumis callosus, Diabetes, α-amylase, α-glycosidase, Antimicrobial.
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