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Delta-Aminolevulinic Acid Dehydratase Assay
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δ-Aminolevulinic acid (ALA) dehydratase catalyzes the synthesis of porphobilinogen
(PBG) from two molecules of ALA. A semimicro method for the colorimetric determination
of ALA dehydratase is presented and applied to various tissues. The enzyme activity in
adult male rat liver was 2.22 and 1.94 μmol PBG formed/g liver/h for homogenates assayed
with or without dithiothreitol, respectively. The assay was linear for at least 2.5 h and for up
to 2.5 mg tissue per assay. The K(m) for ALA was 4.0 X 10^-4 M and the pH optimum was
6.2-6.4. The effects of activators and inhibitors on enzyme activity are discussed.
Title: Delta-Aminolevulinic Acid Dehydratase Assay
Description:
δ-Aminolevulinic acid (ALA) dehydratase catalyzes the synthesis of porphobilinogen
(PBG) from two molecules of ALA.
A semimicro method for the colorimetric determination
of ALA dehydratase is presented and applied to various tissues.
The enzyme activity in
adult male rat liver was 2.
22 and 1.
94 μmol PBG formed/g liver/h for homogenates assayed
with or without dithiothreitol, respectively.
The assay was linear for at least 2.
5 h and for up
to 2.
5 mg tissue per assay.
The K(m) for ALA was 4.
0 X 10^-4 M and the pH optimum was
6.
2-6.
4.
The effects of activators and inhibitors on enzyme activity are discussed.
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