Effect of Moringa Oleifera Leaf Extract on Oxidative Stress and Theca Cell in Polycystic Ovary Syndrome Model with Insulin Resistance

Background: The use of Moringa oleifera as an antioxidant should be investigated as an alternativetreatment of oxidative stress and follicular refinement in PCOS with insulin resistance.Purpose: We aimed to prove the effect of Moringa oleifera leaf extract in various dosages to decrease themalondialdehyde levels and theca cell thickness of PCOS female rat with insulin resistance.Method: Three month old Rattus norvegicus stran wistar rat weighing 100-130 grams were divided into 5groups (n = 8). PCOS model obtained by giving injection of testosterone propionate for 28 days, followedby metformin therapy and Moringa oleifera leaf extract at 250 and 500 mg/KgBW for 14 days. Then, weanalyzed levels of malondialdehyde in the blood and the thickness of theca cell.Results: Malondialdehyde levels in the PCOS control group (5.694±1.464) increased significantly (p <0.05)compared to the normal controls (1.939 ±0.341). Leaf extract Moringa oleifera 500 mg/KgBW (1.982±0.383)showed a significant decrease (p <0.05) to malondialdehyde levels compared to the PCOS control group.Examination of ovarium histology showed that leaf extract Moringa oleifera 500 mg/KgBW (0.931±0.457)significantly decreased the thickness of theca cells (p <0.05) compared to the PCOS control group.Conclusion: Moringa oleifera leaf extract as an antioxidant proven to decrease the malondialdehyde levelsand the thickness of theca cell of the female rat model of PCOS.