Abstract 1400: C1GALT1 regulates malignant phenotypes of cholangiocarcinoma cells

Abstract Identifying molecular targets for cholangiocarcinoma is an urgent need to overcome the treatment failure and death from cancer cell invasion and metastasis. Core 1 β1,3-galactosyltransferase (C1GALT1) has been documented as the key enzyme in morphogenesis and cellular adhesion, for it adds galactose to form Gal-GalNAc-Ser/Thr structure (T antigen); whereas abnormal C1GALT1 expression and aberrant O-glycans are commonly associated with tumor progression. However, functional roles of C1GALT1 in cholangiocarcinoma remains unclear. With clinical correlation, the immunohistochemistry confirmed that higher expression of C1GALT1 in cancer tissue is associated with larger tumor size, advanced cell grade and overall staging. Consistently, stable knockdown of C1GALT1 regarding cholangiocarcinoma cell lines inhibits cell viability, migration, and invasion; whereas the overexpressed C1GALT1 promotes cell viability, migration, and invasion of cholangiocarcinoma cell lines. Additionally, control of C1GALT1 regulates the colony formation, as well as sphere formation of cholangiocarcinoma cells. Moreover, our data suggest that down-regulated C1GALT1 modifies AAL, VVA, PNA, LEL, and MALII lectin, in correlation with the cell malignant behaviors. Given that, C1GALT1 is sufficient to regulate malignant behaviors of cholangiocarcinoma cells with a significant correlation between gene expression and clinical prognosis, implying that C1GALT1 plays a crucial role in cancer progress of cholangiocarcinoma. Citation Format: Po-Da Chen, Ai-An Chang, Min-Chuan Huang, Yao-Ming Wu. C1GALT1 regulates malignant phenotypes of cholangiocarcinoma cells [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2019; 2019 Mar 29-Apr 3; Atlanta, GA. Philadelphia (PA): AACR; Cancer Res 2019;79(13 Suppl):Abstract nr 1400.