Isolation and Purification of Endogenous Microorganisms from Shengli Lignite and Study on Its Ability to Dissolve Lignite

Abstract In order to make more effective use of the abundant lignite resources and to explore more possibilities for the application of lignite, this study describes the isolation of microorganisms from lignite and their dissolution and transformation effects on lignite. Microorganisms were isolated from Shengli lignite using the dilution plating method and the streak plate method, and their species identification was conducted. The lignite dissolution capability of each bacterial strain was tested. The research found that a total of 31 indigenous microorganisms were isolated from Shengli lignite, including 16 bacteria and 15 fungi, among which 9 bacteria and 6 fungi exhibited coal-dissolving effects, with varying degrees of effectiveness. Bacterium SH10 Lysinibacillus fusiformis and fungus L1W Paecilomyces lilacinus showed the best coal-dissolving capabilities. The products of lignite after microbial dissolution were analyzed using gas chromatography-mass spectrometry (GC-MS), revealing that high molecular weight lignite was decom-posed into a variety of small molecular organic compounds during the microbial coal dissolution process. These findings provide a good approach for the green and efficient utilization of lignite, indicating that the decomposition of lignite by microorganisms has a promising prospect for development.