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DNA Synthesis Cycle in Blepharisma*

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SYNOPSIS. Observations made with tritiated thymidine uptake indicate that Blepharisma cannot utilize this precursor directly from the organic medium. An intermediary, such as a food organism (bacteria or Tetrahymena, is needed. These food organisms are not only capable of incorporating thymidine from the medium, but must also break it down or prepare it in a form that is utilizable for Blepharisma.DNA synthesis phase in Blepharisma occurs 17 hours after division. Maximum incorporation is obtained 18 and 19 hours postdivision and lasts thru 24 hours. This is followed by a 1–2 hour postsynthesis phase and division.
Title: DNA Synthesis Cycle in Blepharisma*
Description:
SYNOPSIS.
Observations made with tritiated thymidine uptake indicate that Blepharisma cannot utilize this precursor directly from the organic medium.
An intermediary, such as a food organism (bacteria or Tetrahymena, is needed.
These food organisms are not only capable of incorporating thymidine from the medium, but must also break it down or prepare it in a form that is utilizable for Blepharisma.
DNA synthesis phase in Blepharisma occurs 17 hours after division.
Maximum incorporation is obtained 18 and 19 hours postdivision and lasts thru 24 hours.
This is followed by a 1–2 hour postsynthesis phase and division.

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