Antibacterial Activities of Endophytic Fungi Liquid Cultures from Michelia champaca L. Plant

Endophytic fungi can produce secondary metabolites containing antibacterial compound. Some species of endophytic fungi have been isolated from Michelia champaca L. plant, those are: Colletotrichum alienum, Geotrichum candidum, Rhizoctonia sp, Aspergillus ochraceus, Mycellia sterilia 1, Papulosporasp, Mycellia sterilia 2, Mycellia sterilia 3, Curvularia lunata, Colletotrichum kahawae, dan Aspergillus parasiticus. The aims of this research are: 1) To examine the antibacterial activity of each endophytic fungi species against Escherichia coli, Bacillus subtilis, and Propioni bacterium acnes, 2) to determine the endophytic fungi species that has the highest antibacterial activity. Each endophytic fungi isolate is inoculated on Potato Dextrose Agar plate medium, and incubated in 27oC during 7x24 hours, then cut with a sterile scalpel and inoculated into Potato Dextrose Broth (PDB) medium and shaken at 120 rpm for 7x24 hours. Then the liquid culture was centrifuged at 3000 rpm for 10 minutes. Furthermore, the supernatant from each endophytic fungi was treated to E.coli, B. subtilis and P. acnes to examine the antibacterial activity by agar diffusion method. The research results showed that: 1) each species of endophytic fungi have antibacterial activity against E.coli, B. subtilis and P. acnes, 2) the Rhizoctonia sp. has the highest antibacterial activity against E. coli, Aspergillus parasiticus has the highest antibacterial activity towards B. subtilis, and Curvularia lunata has the highest antibacterial activity towards P. acnes.