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Characteristics of Sialidase in the Rat Salivary Glands
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Using 4-methylumbelliferyl-N-acetylneuraminic acid (4MU-NeuAc) as substrate,
we measured sialidase activity in the salivary glands and other organs of the rat. The
pH optima of salivary gland sialidase were between 4.0 and 4.5, which were similar to those
of the enzyme in the brain, liver and kidney. Among the salivary glands, the submandibular
one showed the highest sialidase activity followed by the parotid and the sublingual glands.
However, sialidase activity in these glands was lower when compared with the activity in the
brain, liver and kidney. From the subcellular distribution study, salivary gland sialidase was
found to be mainly localized in the lysosomes. The pH optima of the lysosomal sialidase of
the salivary glands were between 4.0 and 4.5; and K(m) values for 4MU-NeuAc approximately
0.09 mmol/1. In the submandibular and parotid glands, a soluble sialidase with a different
pH optimum (5.5) and K(m) value (0.25 mmol/1) was also detected.
Title: Characteristics of Sialidase in the Rat Salivary Glands
Description:
Using 4-methylumbelliferyl-N-acetylneuraminic acid (4MU-NeuAc) as substrate,
we measured sialidase activity in the salivary glands and other organs of the rat.
The
pH optima of salivary gland sialidase were between 4.
0 and 4.
5, which were similar to those
of the enzyme in the brain, liver and kidney.
Among the salivary glands, the submandibular
one showed the highest sialidase activity followed by the parotid and the sublingual glands.
However, sialidase activity in these glands was lower when compared with the activity in the
brain, liver and kidney.
From the subcellular distribution study, salivary gland sialidase was
found to be mainly localized in the lysosomes.
The pH optima of the lysosomal sialidase of
the salivary glands were between 4.
0 and 4.
5; and K(m) values for 4MU-NeuAc approximately
0.
09 mmol/1.
In the submandibular and parotid glands, a soluble sialidase with a different
pH optimum (5.
5) and K(m) value (0.
25 mmol/1) was also detected.
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