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Biological Pretreatment Effects on the Bioconversion of Brewer Spent Grain with Hermetia illucens Larvae
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Hermetia illucens is an important species for waste management and the circular economy. The aim of this study was to analyze the effects of Trichoderma reesei C2A and Pleurotus sp. as pretreatments of brewer spent grain (BSG). BSG was inoculated with fungal solution or distilled water (control). After seven days, this was used for H. illucens larvae cultivation. At the end of bioconversion process, parameters of substrate reduction and H. illucens larval development were evaluated. Chemical properties of BSG, frass and larvae were also analyzed. With T. reesei C2A pretreatment, highest substrate reduction (46.3 ± 0.9%) was achieved, but larval growth rate was lower (1.0 ± 0.1 mg/d) than that of control (2.8 ± 0.2 mg/d). Larvae of Pleurotus sp. pretreatment had limited development, reflected in their negative growth rate (−0.6 ± 0.2 mg/d). In conclusion, cultivation of H. illucens larvae (six day old) on BSG pretreated with Pleurotus sp. is not recommended. On the other hand, T. reesei C2A pretreatment enhance BSG reduction, and its potential use for lignocellulosic waste management should be more explored.
Title: Biological Pretreatment Effects on the Bioconversion of Brewer Spent Grain with Hermetia illucens Larvae
Description:
Hermetia illucens is an important species for waste management and the circular economy.
The aim of this study was to analyze the effects of Trichoderma reesei C2A and Pleurotus sp.
as pretreatments of brewer spent grain (BSG).
BSG was inoculated with fungal solution or distilled water (control).
After seven days, this was used for H.
illucens larvae cultivation.
At the end of bioconversion process, parameters of substrate reduction and H.
illucens larval development were evaluated.
Chemical properties of BSG, frass and larvae were also analyzed.
With T.
reesei C2A pretreatment, highest substrate reduction (46.
3 ± 0.
9%) was achieved, but larval growth rate was lower (1.
0 ± 0.
1 mg/d) than that of control (2.
8 ± 0.
2 mg/d).
Larvae of Pleurotus sp.
pretreatment had limited development, reflected in their negative growth rate (−0.
6 ± 0.
2 mg/d).
In conclusion, cultivation of H.
illucens larvae (six day old) on BSG pretreated with Pleurotus sp.
is not recommended.
On the other hand, T.
reesei C2A pretreatment enhance BSG reduction, and its potential use for lignocellulosic waste management should be more explored.
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