The role of the antioxidant defense system in the pathogenesis of changes in protein metabolism during liver ischemia (Preprint)

BACKGROUND To clarify the pathogenesis of changes in protein metabolism in the setting of liver ischemia and to study the role of the antioxidant defense system. OBJECTIVE To clarify the pathogenesis of changes in protein metabolism in the setting of liver ischemia and to study the role of the antioxidant defense system. METHODS It is important to clarify the role of the antioxidant defense system in the pathogenesis of changes in blood protein metabolism depending on the duration of liver ischemia in ag rats, a model of chronic toxicosis.The studies were conducted at the Scientific Research Center of Azerbaijan Medical University using 85 white rats divided into 4 groups. The concentration of total protein, albumin, α1, α2, β, γ globulins, and LDH activity were determined in the blood samples collected from the white rats using reagent kits manufactured by Roche on a Bioscreen MS-2000 microanalyzer. RESULTS Chronic intoxication was created using 20% hydrochloric acid (HCl). To create an ischemia model against its background, the abdominal cavity was opened under anesthesia, and the artery leading to the liver was ligated. Blood was taken from the experimental animals on the 3rd, 7th, 15th, and 30th days, 5 heads each. In order to strengthen the antioxidant defense system, 0.2 ml of riditox solution was injected into the tail vein of the 4th-5th groups of animals once a day for 7 days. The obtained quantitative indicators were statistically processed using a non-parametric method, taking into account modern recommendations CONCLUSIONS The results of our experiments showed that, following the induction of the ischemia model and treatment, several changes occurred in blood protein metabolism. LDH activity increased, while the concentrations of total protein and albumin decreased. The levels of α1- and α2-globulins varied—increasing in some cases and decreasing in others—when compared to both the intact condition and chronic intoxication.