Study of genotypes for clone candidates of high-demand varieties

Abstract. The work is devoted to a highly topical area – domestic clone breeding, as well as the rehabilitation of isolated plants from harmful objects identified in them. Agrobiological records and observations of genotypes for clone candidates of Aligote, Chardonnay and Saperavi varieties identified in the previous period were performed. An assessment of the technological qualities of the yield of isolated plants, a physico-chemical and organoleptic assessment of wine samples obtained from grape plants for clone candidates (harvest 2023) was carried out. The variability/stability of the main agrobiological characteristics and indicators of wine quality was assessed depending on the research period and candidate plants for Aligote A8-14, Chardonnay Sh 1-24(3), Sh 5-22(1), Sh 7- 43(3), Saperavi S6 and S2 clones were identified according to a set of positive signs for further reproduction and evaluation of their vegetative offspring in order to analyze the stability of the identified positive differences from the main varieties. The ampelographic characteristics of the samples, potential clones of the variety, are described. A protocol for the propagation and improvement of grapes by the meristemic method in vitro has been developed (SOP 00668034-015-2024 Standard operating procedure for the propagation and improvement of grapes by the meristemic method in vitro). The identification of phytopathogens in the obtained healthy grape plants was performed. No GLRaV-1 and -3 viruses were detected in plants obtained from genotypes for clone candidates Sh1- 24(3) and S8. In plants from Sh2-9(4), Sh2-5(1) and Saperavi S2, GLRaV-3 was detected in 50, 70 and 40 % of the samples, respectively. In plants of the Aligote A1-20 clone, GLRaV-1 was detected in 60 % of the samples. The phytoplasm Ca. P. solani was not detected in the plants of the Chardonnay clone Sh2-9(4). In vitro genotyping of healthy and propagated clone candidate genotypes was performed at 9 microsatellite (SSR) loci (VVS2, VVMD5, VVMD7, VVMD25, VVMD27, VVMD28, VVMD32, VrZAG62, and VrZAG79). Based on the results of DNA profiling, DNA passports of recovered and in vitro-propagated clone candidate genotypes have been created. Keywords: grapes, clone breeding, productivity, quality, health improvement