Chemical Constituents from Leaves of Gardenia sootepensis and Pseudomussaenda flava Biological Activity and Antioxidant Activity

Abstract The chemical constituents of hexane and ethyl acetate extracts of Gardenia sootepensis (G. sootepensis) and ethyl acetate extract of Pseudomussaenda flava (P. flava) were analyzed for the first time using GC and GC-MS. For the hexane extract of G. sootepensis: Nineteen compounds were identified constituting of 74.70% of the total chromatographical fraction components. The principle compounds were 9, 12, 15-octadecatrienoic acid (23.10%) and squalene (16.50%). For the ethyl acetate extract of G. sootepensis: Sixteen compounds were identified. The main compounds were octadecane (15.40%) and eicosane (14.50%). The ethyl acetate extract of P. flava: Nine compounds were identified. The founded compounds were squalene (21.20%) and 9, 12, 15-octadecatrienoic acid (17.40%). The in vitro antibacterial activity of the leave extracts in various solvents against four bacterial strains were investigated. The hexane and ethyl acetate extracts of G. sootepensis were found to possess antibacterial activity against S. aureus and S. pyogenes with the MIC value of 10 mg/mL respectively. The hexane and ethyl acetate extracts of G. sootepensis showed significant cytotoxicity against NCI-H187 cell lines with the IC50 values of 2.25 and 2.21 µg/mL respectively. But the extracts were non-cytotoxic to MCF-7 cell line. The results revealed that all the medicinal plant extracts possessed antioxidant activity. The ethyl acetate extract of G. sootepensis and P. flava exhibited the highest antioxidant activity with the IC50 value of 6.36 ± 0.02 mg/mL. and 9.74 ± 0.09 mg/mL. Keywords: Antioxidant activity, Biological activity, Chemical constituents, Gardenia sootepensis, Pseudomussaenda flava, Leaves extracts