Search engine for discovering works of Art, research articles, and books related to Art and Culture
Javascript must be enabled to continue!
Feedback loops in the Plk4–STIL–HsSAS6 network coordinate site selection for procentriole formation
View through CrossRef
ABSTRACT
Centrioles are duplicated once in every cell cycle, ensuring the bipolarity of the mitotic spindle. How the core components cooperate to achieve high fidelity in centriole duplication remains poorly understood. By live-cell imaging of endogenously tagged proteins in human cells throughout the entire cell cycle, we quantitatively tracked the dynamics of the critical duplication factors: Plk4, STIL and HsSAS6. Centriolar Plk4 peaks and then starts decreasing during the late G1 phase, which coincides with the accumulation of STIL at centrioles. Shortly thereafter, the HsSAS6 level increases steeply at the procentriole assembly site. We also show that both STIL and HsSAS6 are necessary for attenuating Plk4 levels. Furthermore, our mathematical modeling and simulation suggest that the STIL-HsSAS6 complex in the cartwheel has a negative feedback effect on centriolar Plk4. Combined, these findings illustrate how the dynamic behavior of and interactions between critical duplication factors coordinate the centriole-duplication process.
This article has an associated First Person interview with the first author of the paper.
The Company of Biologists
Title: Feedback loops in the Plk4–STIL–HsSAS6 network coordinate site selection for procentriole formation
Description:
ABSTRACT
Centrioles are duplicated once in every cell cycle, ensuring the bipolarity of the mitotic spindle.
How the core components cooperate to achieve high fidelity in centriole duplication remains poorly understood.
By live-cell imaging of endogenously tagged proteins in human cells throughout the entire cell cycle, we quantitatively tracked the dynamics of the critical duplication factors: Plk4, STIL and HsSAS6.
Centriolar Plk4 peaks and then starts decreasing during the late G1 phase, which coincides with the accumulation of STIL at centrioles.
Shortly thereafter, the HsSAS6 level increases steeply at the procentriole assembly site.
We also show that both STIL and HsSAS6 are necessary for attenuating Plk4 levels.
Furthermore, our mathematical modeling and simulation suggest that the STIL-HsSAS6 complex in the cartwheel has a negative feedback effect on centriolar Plk4.
Combined, these findings illustrate how the dynamic behavior of and interactions between critical duplication factors coordinate the centriole-duplication process.
This article has an associated First Person interview with the first author of the paper.
Related Results
Feedback loops in the Plk4–STIL–HsSAS6 network coordinate site selection for procentriole formation
Feedback loops in the Plk4–STIL–HsSAS6 network coordinate site selection for procentriole formation
SUMMARYCentrioles are duplicated once in every cell cycle, ensuring the bipolarity of the mitotic spindle. Although the core components have been identified, how they cooperate to ...
Characterization of dislocation loops in hydrogen-ion irradiated vanadium
Characterization of dislocation loops in hydrogen-ion irradiated vanadium
Vanadium alloys are considered as the candidate materials for structure application in fusion reactors because of their low radiation-induced activation, high resistance to radiati...
First person – Daisuke Takao
First person – Daisuke Takao
ABSTRACT
First Person is a series of interviews with the first authors of a selection of papers published in Biology Open, helping early-career researchers promote t...
Written Feedback In Second Language Writing: Perceptions Of Vietnamese Teachers And Students
Written Feedback In Second Language Writing: Perceptions Of Vietnamese Teachers And Students
<p>Writing can be very challenging for ESL students since they need to overcome the changes associated with academic writing styles and their mechanics in order to improve th...
Gene Expression Plasticity Is Associated with Regulatory Complexity but Not with Specific Network Motifs
Gene Expression Plasticity Is Associated with Regulatory Complexity but Not with Specific Network Motifs
Over the past two decades, a large body of theoretical and empirical work has been conducted with the aim of identifying the gene regulatory network topologies responsible for gene...
An empirical investigation of contemporary performance management systems
An empirical investigation of contemporary performance management systems
This dissertation provides a comprehensive empirical analysis of contemporary performance management systems (PMS), with a focus on how evolving feedback practices—particularly nar...
Potbellies, wasp‐waists, and superparamagnetism in magnetic hysteresis
Potbellies, wasp‐waists, and superparamagnetism in magnetic hysteresis
Because the response of a magnetic substance to an applied field depends strongly on the physical properties of the material, much can be learned by monitoring that response throug...