A59 A strategy to studying zoonotic infectious diseases

Abstract An increasing number of zoonotic viruses have been detected in animals, especially in poultry species. Understanding the diversity of zoonotic infections and the local behavior helps to characterize the pathogen diversity in human and animals and predict the risk of pathogen spill-over from animals to human. Vietnam is considered, along with other countries in Southeast Asia, as a hotspot for zoonotic viruses. In Vietnam, domestic animals are typically farmed in close proximity to humans, which may increase the risk of transmission of zoonotic pathogens. Our previous studies found the presence of some zoonotic viruses (e.g. rotavirus group A, hepatitis E virus) in domestic pigs. However, the risk of pathogenic transmission from domestic animals to humans has not been determined. Detailed genomic sequence data may help to track the origin and evolution of zoonotic pathogens. To understand the origins and emergence of zoonotic infections in people, who have regular contact with animals, we will investigate the viral diversity in farmers and domestic animals in their farm, using high-throughput sequencing technique. Viral RNA was extracted from pooled fecal samples of 30 farmers and 50 pigs, and used as input for SureSelect target enrichment and Illumina MiSeq sequencing.