Taxonomic description and genome sequence of Rheinheimera mesophila sp. nov., isolated from an industrial waste site

A novel Gram-staining-negative gammaproteobacterium, designated IITR-13T, was isolated from a pesticide-contaminated soil and characterized using a polyphasic approach. On the basis of 16S rRNA gene sequence analysis, the strain showed the closest similarity (98.7 %) toRheinheimera tangshanensisJA3-B52Tfollowed byRheinheimera texasensisA62-14BT(97.7 %) andRheinheimera soliBD-d46T(97.3 %). The 16S rRNA gene sequence similarity of the novel strain to other members of the genusRheinheimerawas < 97.3 %. However, DNA–DNA hybridization between strain IITR-13Tand the type strains ofR. tangshanensis,R. texasensisandR. soliwas 47.5 ± 0.6, 42.4 ± 0.4 and 39.8 ± 0.3 %, respectively; these values are less than 70 %, a threshold value for delineation of a novel species. The strain had C12 : 03-OH, C16 : 0, C17 : 1ω8c, summed feature 3 (C16 : 1ω6c/C16 : 1ω7c) and C18 : 1ω6cas the major fatty acids. The major isoprenoid quinones detected for strain IITR-13Twere ubiquinone Q-8 and menaquinone MK-7.The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and seven unknown phospholipids. Based on phenotypic and chemotaxonomic characteristics and analysis of the 16S rRNA gene sequence, the novel strain should be assigned to a novel species, for which the nameRheinheimera mesophilasp. nov. is proposed, with the type strain IITR-13T( = MTCC 12064T= DSM 29723T). Also, we report the draft genome sequence ofRheinheimera mesophilaIITR-13T; the draft genome sequence includes 3 749 903 bases and comprises 3449 predicted coding sequences, with a G+C content of 47.8 %. It consists of 102 contigs (>1000 bp).