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Association of a RAPD marker with linolenic acid concentration in the seed oil of rapeseed (Brassica napus L.)
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The F2 progeny (64 individuals) from the cross between oilseed rape (Brassica napus L.) cultivar Topas and R4 (a low linolenic mutation line) was analyzed with 8 RFLPs and 34 RAPDs to discover a genetic tag for gene(s) affecting linolenic acid concentration. According to variance analysis (ANOVA), one RAPD marker (25a) was significantly associated with linolenic acid content; the linolenic acid concentration in the seeds of F2 individuals showing the marker (includes both homo- and hetero-zygotes) was 7.43 ± 1.35% and in those lacking the marker was 5.70 ± 1.52%. Marker 25a may be used to facilitate selection for fatty acid composition in future breeding programs of oilseed rape.Key words: Brassica napus, RFLP, RAPD, linolenic acid.
Title: Association of a RAPD marker with linolenic acid concentration in the seed oil of rapeseed (Brassica napus L.)
Description:
The F2 progeny (64 individuals) from the cross between oilseed rape (Brassica napus L.
) cultivar Topas and R4 (a low linolenic mutation line) was analyzed with 8 RFLPs and 34 RAPDs to discover a genetic tag for gene(s) affecting linolenic acid concentration.
According to variance analysis (ANOVA), one RAPD marker (25a) was significantly associated with linolenic acid content; the linolenic acid concentration in the seeds of F2 individuals showing the marker (includes both homo- and hetero-zygotes) was 7.
43 ± 1.
35% and in those lacking the marker was 5.
70 ± 1.
52%.
Marker 25a may be used to facilitate selection for fatty acid composition in future breeding programs of oilseed rape.
Key words: Brassica napus, RFLP, RAPD, linolenic acid.
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