Development and Validation of Uv- Spectrophotometric Method for Estimation of Jatamansone in Nardostachys Jatamansi

Aim: This study aimed to develop and validate a simple, precise, and cost-effective UV- visible spectrophotometric method for the estimation of jatamansone in Nardostachys jatamansi extract. The analysis parameters were selected following the ICH Q2 (R1) guideline. Methods: Jatamansone solution was scanned across the UV-visible range to identify its wavelength of maximum absorbance. Calibration standards of jatamansone were prepared, and a concentration vs. absorbance calibration curve was plotted. The developed UV-visible spectrophotometric method was utilized to estimate jatamansone in standardized extracts of Nardostachys jatamansi. Results: The maximum wavelength of jatamansone was determined to be 278nm. The developed UVvisible spectrophotometric method exhibited a correlation coefficient of 0.999 over a concentration range of 1-6 μg/ml. Validation of the method included assessments of linearity, accuracy, precision, robustness, ruggedness, limit of detection, and limit of quantitation. The developed UV-visible method demonstrated good precision for both intra- and inter-day studies, with standard deviations ranging from 0.75 to 1.798 and 0.889 to 1.572, respectively. The content of jatamansone in the Nardostachys jatamansi extract was found to be 4.72% using the proposed UV-visible spectrophotometry method. Conclusion: A simple, precise, and cost-effective UV-visible spectrophotometric method was successfully developed for the estimation of jatamansone in standardized extracts of Nardostachys jatamansi. The method utilized a solvent with an economical percentage of the organic phase in aqueous media. The validated UVvisible method can be efficiently employed for the estimation of jatamansone in Nardostachys jatamansi extracts.