Antibacterial Activity of Organic Extracts of Root Bark of Ziziphusjujube Gaertn(L) var. hysudrica Edgew

Extracts of root bark of Ziziphus jujubeGaertn(L) var. hysudricaEdgew were prepared by using organic solvents having different polarity i-e methanol, acetone, ethyl acetate, dichloromethane, chloroform and n-hexane. Extracts were then subjected to various characterization studies including UV-Visible and IR profiling. UV-Visible spectrum has shown multiple peaks for all the extracts in UV region. Dichloromethane and chloroform extracts have shown similar pattern as compared to the other extracts. IR spectrum has shown strong –OH (3336.0 cm⁻ˡ) band for methanol and acetone extracts whereas ethylacetate, dichloromethane, chloroform and n-hexane extracts have shown distinct C-N (1250-1020 cm⁻ˡ) stretch band for amines. All the extracts except n-hexane as it was less in yield were screened for antibacterial potential against two gram negative strains (Eescherchia coli and Pseudomonas aeruginosa) and two gram positive strains(Bacillus subtilis and Bacillus pumilus) by using disc diffusion assay. Maximum activity was shown by ethylacetate extract against Eescherchia coli and Bacillus pumilus having zone of inhibition 13.66 mm and 12 mm respectively. Dichloromethane extract has shown high activity against Pseudomonas aeruginosa having zone of inhibition 10.66 mm. chloroform extract was high in term of antibacterial activity against Bacillus subtilis having zone of inhibition 12 mm. data was statistically analyzed by using one-way ANOVA p value less than 0.05 was considered significant. Multiple comparisonswere performed by using LSD test.