Search engine for discovering works of Art, research articles, and books related to Art and Culture
Javascript must be enabled to continue!
Expression of Interleukin‐18 and its Receptor in Mouse Ovary
View through CrossRef
PROBLEM: Interleukin‐18 (IL‐18) strongly induces interferon‐gamma production and is produced not only by types of immune cells but also by types of non‐immune cells. Ovulation is thought to be an inflammation‐like reaction in which many pro‐inflammatory cytokines are involved. We investigated whether IL‐18 is involved in the functions of ovary.
METHOD OF STUDY: The 4‐week‐old immature female mice were examined for IL‐18 and IL‐18 receptor (IL‐18R) expression on their ovaries under stimulation with pregnant mare's serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG) by immunohistochemical staining, Western blotting and reverse transcript‐polymerase chain reaction. The IL‐18R was blocked by the injection of anti‐IL‐18R monoclonal antibody to immature mice during PMSG‐hCG stimulation, and the number of ovulated ova was counted.
RESULTS: The expression of both proteins and mRNA of IL‐18 and IL‐18R were very low in immature ovaries before stimulation, but after PMSG injection both IL‐18 and IL‐18R increased dramatically in theca cells and reached a maximum level at the peri‐ovulatory phase then slightly lowered, but still kept a high level during the luteal phase in the corpus luteum.
The treatment of IL‐18R monoclonal antibody to the mice during ovarian stimulation reduced the number of ovulated ova and inhibited the expansion of cumulus cells surrounding the ovum.
CONCLUSION: IL‐18 and IL‐18R play roles in various kinds of function of ovary.
Wiley
Title: Expression of Interleukin‐18 and its Receptor in Mouse Ovary
Description:
PROBLEM: Interleukin‐18 (IL‐18) strongly induces interferon‐gamma production and is produced not only by types of immune cells but also by types of non‐immune cells.
Ovulation is thought to be an inflammation‐like reaction in which many pro‐inflammatory cytokines are involved.
We investigated whether IL‐18 is involved in the functions of ovary.
METHOD OF STUDY: The 4‐week‐old immature female mice were examined for IL‐18 and IL‐18 receptor (IL‐18R) expression on their ovaries under stimulation with pregnant mare's serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG) by immunohistochemical staining, Western blotting and reverse transcript‐polymerase chain reaction.
The IL‐18R was blocked by the injection of anti‐IL‐18R monoclonal antibody to immature mice during PMSG‐hCG stimulation, and the number of ovulated ova was counted.
RESULTS: The expression of both proteins and mRNA of IL‐18 and IL‐18R were very low in immature ovaries before stimulation, but after PMSG injection both IL‐18 and IL‐18R increased dramatically in theca cells and reached a maximum level at the peri‐ovulatory phase then slightly lowered, but still kept a high level during the luteal phase in the corpus luteum.
The treatment of IL‐18R monoclonal antibody to the mice during ovarian stimulation reduced the number of ovulated ova and inhibited the expansion of cumulus cells surrounding the ovum.
CONCLUSION: IL‐18 and IL‐18R play roles in various kinds of function of ovary.
Related Results
Relation between Interleukin-6, Interleukin-10 and Interleukin-2 Receptor and mortality in Severely Ill COVID-19 patients
Relation between Interleukin-6, Interleukin-10 and Interleukin-2 Receptor and mortality in Severely Ill COVID-19 patients
Objective: To investigate the relation involving soluble interleukin-2 receptor, interleukin-6 and interleukin-10 in hospitalised patients with severe coronavirus disease-2019 infe...
The discriminative role of angiopoietin-like protein-3 for metabolic syndrome in polycystic ovary syndrome
The discriminative role of angiopoietin-like protein-3 for metabolic syndrome in polycystic ovary syndrome
SUMMARY OBJECTIVE: Patients with polycystic ovary syndrome face an increased risk of developing metabolic syndrome. Identifying biomarkers that can detect metabolic syndrome in po...
Rediscovering the rete ovarii, a secreting auxiliary structure to the ovary
Rediscovering the rete ovarii, a secreting auxiliary structure to the ovary
The
rete ovarii
(RO) is an appendage of the ovary that has been given little attention. Although the RO appears in drawings of the ovary in ...
Rediscovering the rete ovarii, a secreting auxiliary structure to the ovary
Rediscovering the rete ovarii, a secreting auxiliary structure to the ovary
The
rete ovarii
(RO) is an appendage of the ovary that has been given little attention. Although the RO appears in drawings of the ovary in ...
Mutations in Insulin-Receptor Gene in Insulin-Resistant Patients
Mutations in Insulin-Receptor Gene in Insulin-Resistant Patients
Defects in insulin-receptor function have been associated with insulin-resistant states such as obesity and non-insulin-dependent diabetes mellitus (NIDDM). Several types of mutati...
THE STUDY OF APOPTOSIS MECHANISM IN THE PROCESS OF RECOMBINANT INTERLEUKIN-12 TREAT THE MICE WITH VIRAL MYOCARDITIS
THE STUDY OF APOPTOSIS MECHANISM IN THE PROCESS OF RECOMBINANT INTERLEUKIN-12 TREAT THE MICE WITH VIRAL MYOCARDITIS
Objectives
Through studying the NK cell activity, the pathological changes of myocardial cells and the changes of apoptosis in the process of recombinant interleu...
CB2/miR-124 signaling down-regulate the expression of purinergic P2X4 and P2X7 receptor in dorsal spinal cord of CCI rats
CB2/miR-124 signaling down-regulate the expression of purinergic P2X4 and P2X7 receptor in dorsal spinal cord of CCI rats
Abstract
Background: The importance of P2X purinoceptors, CB2 receptor and microRNA-124(miR-124) in spinal cord microglia to the development of neuropathic pain was demonst...
Rediscovering the Rete Ovarii: a secreting auxiliary structure to the ovary
Rediscovering the Rete Ovarii: a secreting auxiliary structure to the ovary
Abstract
The rete ovarii (RO) is an appendage of the ovary that has been given little attention. Although the RO appears in drawings of the ovary in early versions ...