IN-VIVO ANALYSIS OF OXIDATIVE STRESS IN AESTHETIC COATED ORTHODONTIC ARCH-WIRES & BRACKETS

Objectives: To assess in vivo the oxidative damage that aesthetic coated arch-wires causes by measuring 8OHdG (8-hydroxydeoxyguanosine ) as an oxidative marker to detect the level of oxidative stress generation.Materials and Methods: About 80 patients of ages 15-30 years enrolled in the study having fi xed orthodontic treatment were divided into control (patients with NiTi wire) and experimental groups including patients with wires coated in PTFE, epoxy resin, and rhodium. Saliva samples were collected with an unstimulated saliva collection method at two intervals. The first sample was collected before the bonding process and insertion of the arch-wires and the second sample was collected four weeks after the insertion of arch-wires. The samples were kept in storage at -80°C, and the amounts of 8-OHdG were measured by ELISA analysis.Results: The salivary 8-OhdG level in all groups was measured at day 0 and the end of week 4. At day 0, It was 1.60±43 ng/ml in the NiTi group,1.60±0.52 ng/ml in the epoxy resin group, 1.70±0.12 ng/ml in the rhodium group and 1.63±0.40 ng/ml in PTFE group. After 4 weeks, it was 3.6810.47 ng/ml in the NiTi group, 3.50±0.77 ng/ml in the epoxy resin group, 3.06±0.70 ng/ml in the rhodium group and 2.75±0.72 ng/ml in PTFE group, p = 0.812) This demonstrated that while there was no noteworthy variation in the groups' mean 8-OHdG levels at day 0, there was at week 4. Post hoc Tukey test revealed that PTFE-coated arch-wires and Rhodium group had significantly lower 8-OHdG levels compared to NiTi and Epoxy resin-coated arch-wire. Salivary 8-OHdG levels increased significantly after 4 weeks of arch-wire use in all groups.Conclusion: This study suggests that PTFE-coated arch-wires are the most biocompatible among the coated arch-wires studied.