PpMYB105 inhibited chilling injury by regulating PpMsrA1 in peach fruit

Abstract Cold storage can maintain the quality of postharvest fruit. However, peaches are easy to suffer from chilling injury during cold storage, leading to economic loss. Our preliminary research has demonstrated that MeJA supplementation reduced the chilling injury (CI) severity, and enhanced the methionine sulfoxide reductase A1 (PpMsrA1) expression. In this study, results showed that MeJA application elevated the MsrA activity and methionine (Met) content, and reduced the methionine-S-sulfoxide (Met-S-SO) content and reactive oxygen species (ROS) production. Moreover, PpMYB105, isolated by yeast one-hybrid screening, could activate the transcription of PpMsrA1 by binding to the MYB binding element in its promoter. The gene expression of PpMYB105 was up regulated by MeJA application. Overexpression of PpMYB105 in tomatoes enhanced the chilling tolerance and gene expression of SlMsrA1. Virus-induced gene silencing (VIGS) of PpMYB105 in peaches resulted in the increase in CI severity and the decrease in gene expression of PpMsrA1. Thus, PpMYB105 was involved in the MeJA-boosted chilling tolerance by regulating PpMsrA1.