Wound Healing Activity of Alari Flower (Nerium oleander linn) Powder on Excised Wound in Wistar Albino Rats– A Five Arm Randomized Controlled Study

Wound healing is a dynamic and intricate biological process that plays a pivotal role in tissue repair and regeneration. There are several topical medications used for wound healing. Anyhow their efficacy is challenged because of development of bacterial resistant. This study aimed to evaluate the wound healing efficacy on excised wound with Nerium oleander flower powder in Wistar albino rats. Study was conducted by using, healthy albino rats. They were grouped into negative Control, positive control I, positive control II, Test Drug I and Test Drug II. Groups with each comprising of six rats. A wound size of 120mm2 was inflicted on the dorsolateral aspect of the thorax of the rats. Negative Control group left untreated, positive control I group received Neosporin powder, Test Drug 1 received Nerium oleander flower powder, positive control II received 5 % Neosporin ointment, Test Drug II received Nerium oleander flower powder mixed with ointment base. Until the 14th day, the wound's size was measured once every two days. The proportion of the wound contraction was calculated as a percentage change in the original size of the wound across different days. Based on the results of the Bates Jensen Wound Assessment tool, the parameters of the wound edges, exudate type, exudate amount, and epithelialization are used for the overall evaluation of the wound. One-way ANOVA in SPSS was used for the statistical analysis. Results shows value< 0.05 with 95% of confidence interval was noted in all Test groups. Test Drug 2 shows 83% of wound contraction, and Test Drug 1 shows 74% contraction. Positive control I shows 80% and positive control II shows 85%. Negative Control group shows 55% of wound contraction. Further, Test groups establishes a good grip on the wound edges, early reduction of exudates amounts and causing shorter epithelialization time in the wounds compare than Standard group. It is concluded that flower powder of Nerium oleander is moderately significant, and ointment form has highly significant wound healing effect in excised wounds.