F5 6665A>G Polymorphism Is Associated with Increased Risk of Venous Thromboembolism in Females

The main goal of our study was to assess the potential effect of the polymorphism of the coagulation-related genes F2, F5, and F13A on the risk of venous thromboembolism (VTE) development. The study was conducted at the Clinical Center, Podgorica, Montenegro, and included 103 VTE patients and 106 sex- and age-matched healthy controls. Demographic, clinical, and laboratory data were obtained from the medical records and questionnaires. Genotyping for F2 19911A>G (rs3136516), F5 6665A>G (rs6027), and F13A 102G>T (rs5985) was performed by allele-specific PCR. Controlling for the effect of known risk factors, the presence of at least one variant F5 6665 G allele conferred a significantly higher risk of VTE among females [OR (95%CI): 64.06 (5.38; 763.61)], but not among males. In addition, thromboembolic events were associated with comorbidities [OR (95%CI): 197.10 (19.17; 2026.19)], overweight [OR (95%CI): 33.59 (2.47; 456.65)], and the presence of F2 20210G>A [OR (95%CI): 32.43 (4.21; 249.77)] and F5 1601G>A [OR (95%CI): 144.80 (13.59; 1542.63)] in females, as well as with comorbidities [OR (95%CI): 6.32 (1.90; 20.98)], family history of VTE [OR (95%CI): 8.10 (2.28; 28.83)], and the presence of F5 1601G>A [OR (95%CI): 20.10 (2.34; 173.02)] in males. Our study reports an association between the presence of at least one F5 6665G variant allele and an increased risk of VTE development in females. Our results indicate that F5 6665A>G, in combination with other confirmed factors of influence, such as comorbidities, overweight, F2 20210G>A, and F5 1601G>A, could contribute to VTE risk prediction in females.