The influence of carbofuran insecticide exposure on the number of ovarian follicles in mice (Mus musculus)

Objective: The aim of this research was to investigate the histopathological damage to the ovaries of mice (Mus musculus) caused by exposure to carbofuran. Method: Twenty female mice aged 10 weeks with a body weight ranging from 25-30 mg were used in this study. The mice were divided into four groups, with each group consisting of five replicates. The control group (P0) received 0.5% physiological NaCl, while the treatment groups (P1, P2, and P3) were exposed to carbofuran at doses of 0.0833 mg/kg BW, 0.0417 mg/kg BW, and 0.0208 mg/kg BW, respectively, for ten days. On the 12th day, ovarian necropsy and HE staining were performed to observe the number of primary, secondary, tertiary, and Graafian follicles. Data analysis was conducted using ANOVA and BNJ tests. Result: The results of this research showed that oral administration of carbofuran leads to a decrease in the number of secondary, tertiary, and Graafian follicles. High doses, such as 0.0833 mg/kg body weight, resulted in a more pronounced reduction in the number of secondary, tertiary, and Graafian follicles compared to the control group. Conclusion: A reduction in the number of secondary, tertiary, and Graafian follicles with increasing doses of carbofuran.