Receptor Identification and Characterization

Title: Receptor Identification and Characterization

Description:

Abstract The sections in this article are: Identification of Receptors Based on Functional Properties Schild Analysis Identification of Receptors with Radioligand Binding Choosing the Biological Preparation Choosing a Radioligand Separating Bound From Free Radioligand Irrelevant Binding Establishing That the Radioligand Binding Detected Reflects Interaction With the Physiologically Significant Receptor Analysis of Radioligand Binding Data Based on the Law of Mass Action Fractional Occupancy Assumptions Inherent in the Law of Mass Action Saturation Binding Studies Defining Nonspecific Binding in a Radioligand Binding Assay Defining The Specificity of Radioligand Binding Using Competitive Binding Analysis Analyzing Competitive Binding Data Calculating the K i From the IC 50 Homologous Competitive Binding Curves Permit an Assessment of Both K d and B max Kinetic Analysis of Radioligand Binding Experiments Studies of Radioligand Dissociation Studies of Radioligand Association Complex Binding Phenomena Competitive Binding Experiments With Two (or More) Receptor Sites Saturation Binding Experiments With Two Receptor Sites Comparing One‐ and Two‐Site Models The Slope Factor of a Competitive Binding Curve Using Dissociation Experiments to Investigate Complex Binding Distinguishing Between Independent Receptor Subtypes and Negative Cooperativity Evaluating Allosteric Phenomena Obtaining Independent Data to Clarify the Biological Origin of Complex Binding Phenomena Agonist Binding and the Ternary Complex Summary.