Search engine for discovering works of Art, research articles, and books related to Art and Culture
Javascript must be enabled to continue!
Preparation and Characterization of Monoclonal Antibody Against Translationally Controlled Tumor Protein
View through CrossRef
From our previous proteomic research, we found that translationally controlled tumor protein (TCTP) might play at least a partial role in colon adenocarcinoma progression. However, the precise impact of TCTP on colorectal cancer metastasis progression is currently still unknown. Therefore, immunology reagents are urgently needed to proceed with the next mechanism-related research. Moreover, the identification of TCTP expression level in tissue of colorectal cancer patients also requires substantial amounts of immunology reagents. In this report, monoclonal antibodies (MAbs) against to TCTP were made from hyperimmune Balb/c mice, by injecting 50 μg of purified antigen intraperitoneally. Hybridomas were screened by indirect enzyme-linked immunosorbent assay (ELISA) using purified protein. Finally six mouse hybridomas producing MAbs to TCTP were established. The MAbs obtained were fully characterized using Western blot analysis and immunohistochemistry. The results showed that these antibodies could be used for the preliminary application of the next mechanism-related research and TCTP expression level analysis.
SAGE Publications
Title: Preparation and Characterization of Monoclonal Antibody Against Translationally Controlled Tumor Protein
Description:
From our previous proteomic research, we found that translationally controlled tumor protein (TCTP) might play at least a partial role in colon adenocarcinoma progression.
However, the precise impact of TCTP on colorectal cancer metastasis progression is currently still unknown.
Therefore, immunology reagents are urgently needed to proceed with the next mechanism-related research.
Moreover, the identification of TCTP expression level in tissue of colorectal cancer patients also requires substantial amounts of immunology reagents.
In this report, monoclonal antibodies (MAbs) against to TCTP were made from hyperimmune Balb/c mice, by injecting 50 μg of purified antigen intraperitoneally.
Hybridomas were screened by indirect enzyme-linked immunosorbent assay (ELISA) using purified protein.
Finally six mouse hybridomas producing MAbs to TCTP were established.
The MAbs obtained were fully characterized using Western blot analysis and immunohistochemistry.
The results showed that these antibodies could be used for the preliminary application of the next mechanism-related research and TCTP expression level analysis.
Related Results
7
th
International Symposium on Enabling Technologies for Life Sciences (ETP)
7
th
International Symposium on Enabling Technologies for Life Sciences (ETP)
The seventh in the series of ETP Symposia (see
Rapid Communications in Mass Spectrometry
2012,
26
, ...
Complex Collision Tumors: A Systematic Review
Complex Collision Tumors: A Systematic Review
Abstract
Introduction: A collision tumor consists of two distinct neoplastic components located within the same organ, separated by stromal tissue, without histological intermixing...
The TEMPI syndrome: Unique patient specific monoclonal antibodies as tools for target antigen discovery
The TEMPI syndrome: Unique patient specific monoclonal antibodies as tools for target antigen discovery
Abstract
BACKGROUND. In 2011, we discovered the TEMPI syndrome. TEMPI is a rare monoclonal gammopathy of clinical signif...
Giant Sacrococcygeal Teratoma in Infant: Systematic Review
Giant Sacrococcygeal Teratoma in Infant: Systematic Review
Abstract
Introduction
Sacrococcygeal teratoma (SCT) is a rare embryonal tumor that occurs in the sacrococcygeal region, with an incidence of about 1 in 35,000 to 40,000 live births...
The TEMPI Syndrome: A Monoclonal Gammopathy of Clinical Significance Driving Erythropoietin Production
The TEMPI Syndrome: A Monoclonal Gammopathy of Clinical Significance Driving Erythropoietin Production
BACKGROUND. The TEMPI syndrome is a rare monoclonal gammopathy of clinical significance (MGCS) that we discovered in 2011. Patients with the TEMPI syndrome display five hallmark fe...
Procedure for Western blot v1
Procedure for Western blot v1
Goal: This document has the objective of standardizing the protocol for Western blot. This technique allows the detection of specific proteins separated on polyacrylamide gel and t...
Safety and Efficacy of Atezolizumab in Ovarian Cancer
Safety and Efficacy of Atezolizumab in Ovarian Cancer
Abstract
Introduction
Although the efficacy of PD-L1 blockade has been evaluated in analyses that combine pharmacologically distinct antibodies, the specific efficacy and safety of...
Supplementary Data from Targeted BiTE Expression by an Oncolytic Vector Augments Therapeutic Efficacy Against Solid Tumors
Supplementary Data from Targeted BiTE Expression by an Oncolytic Vector Augments Therapeutic Efficacy Against Solid Tumors
<p>Supplementary Methods, Supplementary Figures S1-S15 Fig. S1. Purification and binding specificity of MV-encoded BiTEs. (A) Purification of MV-expressed BiTEs. Vero cells w...