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Live-Cell Imaging of (p)ppGpp with RNA-based Fluorescent Sensors
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Abstract
Guanosine tetra- and pentaphosphate, (p)ppGpp, are important alarmone nucleotides that regulate bacterial survival in stressful environment. A direct detection of (p)ppGpp in living cells is critical for our understanding of the mechanism of bacterial stringent response. However, it is still challenging to directly image and measure cellular (p)ppGpp. Here, we report a type of RNA-based fluorescent sensors for live-cell imaging of (p)ppGpp. Our sensor is engineered by conjugating a recently identified (p)ppGpp-specific riboswitch with a fluorogenic RNA aptamer, Broccoli. These sensors can be genetically encoded and enable direct monitoring of cellular (p)ppGpp accumulation. Unprecedented information on cell-to-cell variation and cellular dynamics of (p)ppGpp levels can now be obtained under different nutritional conditions. We predict that these RNA-based sensors will be broadly adapted to study bacterial stringent response.
Title: Live-Cell Imaging of (p)ppGpp with RNA-based Fluorescent Sensors
Description:
Abstract
Guanosine tetra- and pentaphosphate, (p)ppGpp, are important alarmone nucleotides that regulate bacterial survival in stressful environment.
A direct detection of (p)ppGpp in living cells is critical for our understanding of the mechanism of bacterial stringent response.
However, it is still challenging to directly image and measure cellular (p)ppGpp.
Here, we report a type of RNA-based fluorescent sensors for live-cell imaging of (p)ppGpp.
Our sensor is engineered by conjugating a recently identified (p)ppGpp-specific riboswitch with a fluorogenic RNA aptamer, Broccoli.
These sensors can be genetically encoded and enable direct monitoring of cellular (p)ppGpp accumulation.
Unprecedented information on cell-to-cell variation and cellular dynamics of (p)ppGpp levels can now be obtained under different nutritional conditions.
We predict that these RNA-based sensors will be broadly adapted to study bacterial stringent response.
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