Gm9795 Promotes Inflammation in Non-Alcoholic Steatohepatitis via NF-κB/JNK Pathway by Endoplasmic Reticulum Stress

Abstract Background: Non-alcoholic steatohepatitis (NASH) is a key stage in leading development of non-alcoholic simple fatty liver (NAFL) into cirrhosis and even liver cancer. This study aimed at exploring the lncRNA expression profifile in NASH and the biological function of a novel LncRNA-gm9795. Methods: Microarray analysis was performed to compare the expression profifiles of lncRNAs in the liver of NASH, NAFLD and normal mice. Methionine-choline-deficient Medium(MCD) with Lipopolysaccharide(LPS) or palmitic acid（PA）were used to built NASH cell models.The role and mechanism of LncRNA-gm9795 in NASH were explored by knocking down or over-expressing its expression. Results: A total of 381 lncRNAs were found to be not only highly expressed in NAFLD, but also is going to go even higher in NASH. A novel LncRNA-Gm9795 was significantly highly expressed in liver tissues of NASH animal models and NASH cell models. By staining with Nile red, we found that Gm9795 did not affect the fat accumulation of NASH. However, Gm9795 in NASH cell models significantly promoted the expression of TNFα, IL-6, IL-1β, the important inflammatory mediators in NASH. At the same time, we found that Gm9795 upregulated the key molecules in endoplasmic reticulum stress (ERS), while NF-κB/JNK pathways were also activated. When ERS activator Thapsigargin(TG) was introduced in cells with Gm9757 si-RNA, NF-κB and JNK pathways were activated. Conversely, ERS inhibitor Tauroursodeoxycholic(TUDCA) acid inhibited NF-kB and JNK pathways in cells with Gm9795 overexpression plasmid. Conclusion: LncRNA-gm9795 promotes inflammatory response in NASH through NF-kB and JNK pathways by ERS, which might provide theoretical basis for revealing the pathogenesis of NASH and discovering new therapeutic targets