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Comparison of Start Codon Targeted (SCoT) and EST-SSR markers in sesame
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Expressed sequence tag (EST)-SSR markers and Start codon targeted (SCoT) markers were compared to estimate genetic diversity among thirty sesame genotypes. To achieve this goal, the available sesame database was used as a resource for the development of novel microsatellite (SSR) and Start codon targeted (SCoT) markers. EST- SSR markers detected a mean of 4.34 alleles with a mean polymorphic information content (PIC) of 0.84, against SCoT markers with a mean of 9.6 alleles and a mean PIC of 0.79. SCoT markers amplified more null alleles than EST-SSRs. Geographical diversity did not corroborate with genotypic diversity as the cluster composition revealed that genotypes belonging to different geographical origin grouped in the same cluster.
Indian Society of Oilseeds Research
Title: Comparison of Start Codon Targeted (SCoT) and EST-SSR markers in sesame
Description:
Expressed sequence tag (EST)-SSR markers and Start codon targeted (SCoT) markers were compared to estimate genetic diversity among thirty sesame genotypes.
To achieve this goal, the available sesame database was used as a resource for the development of novel microsatellite (SSR) and Start codon targeted (SCoT) markers.
EST- SSR markers detected a mean of 4.
34 alleles with a mean polymorphic information content (PIC) of 0.
84, against SCoT markers with a mean of 9.
6 alleles and a mean PIC of 0.
79.
SCoT markers amplified more null alleles than EST-SSRs.
Geographical diversity did not corroborate with genotypic diversity as the cluster composition revealed that genotypes belonging to different geographical origin grouped in the same cluster.
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