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Neurogenesis with iron supplementation and label-free nDIA proteomics v1
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This protocol describes the NGN2-driven differentiation of H9 stem cells into iNeurons ± FAC iron supplementation. The protocol also covers sample preparation of label free proteomics and analysis via nDIA on the Astral Orbitrap mass analyzer.
Title: Neurogenesis with iron supplementation and label-free nDIA proteomics v1
Description:
This protocol describes the NGN2-driven differentiation of H9 stem cells into iNeurons ± FAC iron supplementation.
The protocol also covers sample preparation of label free proteomics and analysis via nDIA on the Astral Orbitrap mass analyzer.
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