IN VITRO ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF ISOLATED FLAVONOID FROM METHANOLIC EXTRACT OF ANDROGRAPHIS ECHIOIDES LEAVES

The objective of this investigation was to identify the active flavonoid present across the entire Andrographis echioides plant. Using a methanolic extract from the leaves of Andrographis echioides, it was examined how flavonoids may be isolated from a plant component. Phytochemical analyses revealed the presence of flavonoid components in methanol extract. The recovered flavonoid component was further purified by column chromatography and TLC. The structures of the isolated compounds were characterized using UV-visible, FT-IR, 1H & 13C NMR and mass spectroscopy. This led to the isolated compound being given the name 9-methoxy-6a,11a-dihydro-6H-benzofuro[3,2-c]chromen-3-ol or medicarpin. Further biological investigation is needed for the isolated flavonoid discovered in this plant. Recent research has concentrated on the role of flavonoids as antioxidants when oxidative stress is present. The antioxidant properties of flavonoids were also discussed.