The chloroplast genome of black currant (DNA.) cultivar 'Kipiana': genome structure and chloroplast Simple Sequence Repeats

The study has been conducted in year 2022-2023 at Russian Research Institute of Fruit Crop Breeding (VNIISPK), 302530 Orel, Russia with help of computational resources provided by the Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia. Ribes nigrum, commonly known as black currant, is a perennial deciduous shrub having an economic importance. Though of economical importance of Ribes nigrum its complete chloroplast genome sequences is not published yet. Here, the complete chloroplast genome of black currant cultivar 'Kipiana' was sequenced using Illumina sequencing technology and de novo assembled for the first time. Simple sequence repeats were detected and located in the chloroplast genome. The plastome is 157,450 bp in length, with a GC content of 38.1%. The chloroplast genome is features a typical quadripartite structure, consisting of a pair of inverted repeat regions of 26,007 bp for each repeat unit, separated by a large single copy region of 87,086 bp, and a small single copy region of 18,350 bp. A total of 133 genes were annotated in the plastome, including 85 protein coding genes, 37 tRNA genes, 8 rRNA genes and 3 pseudogenes (both copies of ycf15 and one copy of ycf1 gene). Totally 66 simple sequence repeats (SSRs) were identified within the assembled Ribes nigrum plastid genome. Thus, our study provides genomic resources and valuable reference for marker development and phylogenomics in Ribes.