Exploration of the chemical constituents of Tricholomopsis aurea (Beeli) Desjardin using UHPLC-ESI-MS/MS and NMR spectroscopy

Abstract Tricholomopsis aurea(Beeli) Desjardin & B.A. Perry is a wild edible fungus that is scarce in Cameroon, but common in other parts of the world. The metabolites of this food matrix have not been extensively analyzed thus far. This work aimed to establish the metabolic profile of Tricholomopsis aurea by UHPLC-ESI-MS/MS and to isolate some of the major compounds using chromatographic separation techniques. Overall, 65 compounds were identified as belonging to 18 classes, including amino acids, carbohydrates, vitamins, aminoglycolipids, phosphoglycolipids, fatty acids, phenolics, nucleosides, steroids, fatty acid amides, acyl glycerides, sphingolipids, alkaloids, terpenes, carotenoids, saponins, aromatic amines, and peptides. Six of the isolated compounds were characterized as oleic acid, elaidic acid petroselinic acid, ergosterol, ergosterol 5α,8α-endoperoxide and adenosine using 1D and 2D NMR spectroscopy. Fatty acids, steroids, amino acids and peptides were the main components of the mushroom. T. aureahas been proven to be a valuable source of chemically diverse compounds and to contain indispensable nutrients, such as amino acids, fats, carbohydrates and vitamins, which are essential for promoting the health span.