Biochemical Application of New Synthetic Compounds of Pyridine Derivatives Incorporating on Tetrazole Moieties

 Introduction: Pyridine has a liquid state, bad odor, pungent taste, toxic effects, and has the ability to mix with alcohol, water and some sperms, and it has many effects in the event of severe exposure such as suffocation. Methods: In this work, a new series of tetrazole derivatives is synthesized by reacting sodium azide with Schiff bases derivatives, which are prepared by condensation reaction between some pyridine derivatives (2-amino- 5-chloropyridine, 2-amino-4-methyl pyridine) and some aromatic aldehydes (dimethyl aminobenzaldehyde, p-aminobenzaldehyde, p-chlorobenzaldehyde, p-bromobenzaldehyde, and salicylic aldehyde). Result: All the resulting compounds were characterized by FT-IR and milting points. The study of the biological activity of Schiff base derivatives and tetrazole derivatives against E. coli shows that all derivatives give positive results at different diameters, but the compounds (2,6,10) give a lower inhibition at concentrations of 100 mg/ml, as well as the compounds (2,3,5,7,9) give a lower inhibition at concentrations of 75 mg/ml. The compounds (6,8,10) give lower inhibition at concentration (100 mg/ml) against S. aureus, as well as the compounds (1,3,6,8,10) give lower inhibition at concentration (75 mg/ml). Conclusion: It was shown during the process of preparing the compounds that the difference in the groups substituted for the same compound leads to a difference in the percentages, reaction time, and biological activity of the resulting compounds.