Search engine for discovering works of Art, research articles, and books related to Art and Culture
Javascript must be enabled to continue!
IT-scATAC-seq v1
View through CrossRef
Single-cell ATAC-seq (scATAC-seq) allows for detailed mapping of chromatin accessibility but often faces challenges related to throughput, cost, and equipment demands. In this study, we introduce indexed Tn5 tagmentation-based scATAC-seq (IT-scATAC-seq), a semi-automated, economical, and scalable method that utilizes indexed Tn5 transposomes along with a three-round barcoding strategy. This workflow can prepare libraries for up to 10,000 cells in just one day, lowers the cost per cell to around $0.01, and preserves strong data resolution.
Title: IT-scATAC-seq v1
Description:
Single-cell ATAC-seq (scATAC-seq) allows for detailed mapping of chromatin accessibility but often faces challenges related to throughput, cost, and equipment demands.
In this study, we introduce indexed Tn5 tagmentation-based scATAC-seq (IT-scATAC-seq), a semi-automated, economical, and scalable method that utilizes indexed Tn5 transposomes along with a three-round barcoding strategy.
This workflow can prepare libraries for up to 10,000 cells in just one day, lowers the cost per cell to around $0.
01, and preserves strong data resolution.
Related Results
Benchmarking Algorithms for Gene Set Scoring of Single-cell ATAC-seq Data
Benchmarking Algorithms for Gene Set Scoring of Single-cell ATAC-seq Data
AbstractGene set scoring (GSS) has been routinely conducted for gene expression analysis of bulk or single-cell RNA-seq data, which helps to decipher single-cell heterogeneity and ...
Semi-automated IT-scATAC-seq profiles cell-specific chromatin accessibility in differentiation and peripheral blood populations
Semi-automated IT-scATAC-seq profiles cell-specific chromatin accessibility in differentiation and peripheral blood populations
Abstract
Single-cell ATAC-seq (scATAC-seq) enables high-resolution mapping of chromatin accessibility but is often limited by throughput, cost, and equipment requirements...
Human-scATAC-Corpus: a comprehensive database of scATAC-seq data
Human-scATAC-Corpus: a comprehensive database of scATAC-seq data
ABSTRACT
Single-cell Assay for Transposase-Accessible Chromatin using sequencing (scATAC-seq) profiles chromatin accessibility at cellular resolution, making it possibl...
MARS-seq2.0: an experimental and analytical pipeline for indexed sorting combined with single-cell RNA sequencing v1
MARS-seq2.0: an experimental and analytical pipeline for indexed sorting combined with single-cell RNA sequencing v1
Human tissues comprise trillions of cells that populate a complex space of molecular phenotypes and functions and that vary in abundance by 4–9 orders of magnitude. Relying solely ...
Generating Synthetic Single Cell Data from Bulk RNA-seq Using a Pretrained Variational Autoencoder
Generating Synthetic Single Cell Data from Bulk RNA-seq Using a Pretrained Variational Autoencoder
AbstractSingle cell RNA sequencing (scRNA-seq) is a powerful approach which generates genome-wide gene expression profiles at single cell resolution. Among its many applications, i...
Abstract P1-05-23: Utilities and challenges of RNA-Seq based expression and variant calling in a clinical setting
Abstract P1-05-23: Utilities and challenges of RNA-Seq based expression and variant calling in a clinical setting
Abstract
Introduction
Variant calling based on DNA samples has been the gold standard of clinical testing since the advent of Sanger sequencing. The u...
IReNA: integrated regulatory network analysis of single-cell transcriptomes and chromatin accessibility profiles
IReNA: integrated regulatory network analysis of single-cell transcriptomes and chromatin accessibility profiles
AbstractAlthough single-cell RNA sequencing (scRNA-seq) and single-cell assay for transposase accessible chromatin using sequencing (scATAC-seq) have been widely used, few methods ...
Global Prediction of Chromatin Accessibility Using RNA-seq from Small Number of Cells
Global Prediction of Chromatin Accessibility Using RNA-seq from Small Number of Cells
ABSTRACT
Conventional high-throughput technologies for mapping regulatory element activities such as ChIP-seq, DNase-seq and FAIRE-seq cannot analyze samples with s...