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Bioactivity Assay of Chitosan Microspheres Containing Pig Thymosin<i> In Vitro</i>
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Thymosin, composed of many peptides, is distributed in animal and has immunomodulating effects. However, many factors could affect its bioactivity. Chitosan microspheres containing pig thymosin (CMPT) were prepared through W/O emulsion cross-linking method. In this study, thymosin activity between pig-thymosin and CMPT was investigated through E-rosette test and mouse spleen lymphocyte proliferation (MPLP) experiment in vitro. Results showed that CMPT has the same immunomodulatory activity as pig thymosin. When pig-thymosin was 1.5 μg / ml, E-rosette binding rate was 63%, and E-rosette binding rate of CMPT reached 60%。MPLP rate was 73.3% and 68.5%, respectively. CMPT have the capacity to keep the E-rosette binding and MPLP activity of pig thymosin. This may help to improve traditional formulation and bioavailability of thymosin, and expand its scope of application in clinic.
Trans Tech Publications, Ltd.
Title: Bioactivity Assay of Chitosan Microspheres Containing Pig Thymosin<i> In Vitro</i>
Description:
Thymosin, composed of many peptides, is distributed in animal and has immunomodulating effects.
However, many factors could affect its bioactivity.
Chitosan microspheres containing pig thymosin (CMPT) were prepared through W/O emulsion cross-linking method.
In this study, thymosin activity between pig-thymosin and CMPT was investigated through E-rosette test and mouse spleen lymphocyte proliferation (MPLP) experiment in vitro.
Results showed that CMPT has the same immunomodulatory activity as pig thymosin.
When pig-thymosin was 1.
5 μg / ml, E-rosette binding rate was 63%, and E-rosette binding rate of CMPT reached 60%。MPLP rate was 73.
3% and 68.
5%, respectively.
CMPT have the capacity to keep the E-rosette binding and MPLP activity of pig thymosin.
This may help to improve traditional formulation and bioavailability of thymosin, and expand its scope of application in clinic.
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