PHARMACOGNOSTICAL, FLUORESCENCE ANALYSIS, HEAVY METAL TESTING AND TLC PROFILING OF THE PLANT BOUGAINVILLEA GLABRA (CHOISY)

Objective: The studys objective is to examine the plant Bougainvillea glabra (Choisy) efficacy by phytochemical, pharmacognostical, fluorescence analysis, heavy metal testing and TLC profiling. Methods: A variety of solvents, including petroleum ether, chloroform, ethyl acetate, methanol, and water, were used to extract the plant powder. To identify different phytochemical ingredients, the various extracts were subjected to qualitative testing. Heavy metal testing and fluorescence examination in daytime and ultraviolet light (254 nm and 365 nm) were performed on the plant powder. For the identification of active phytoconstituents TLC procedure was performed by using various solvent systems. Results: The water soluble extractive value was found to be greater than that of petroleum ether, methanol, ethyl acetate, and chloroform. The values of total ash were found to be greater than those of water-soluble and acid-insoluble ash. Numerous phytochemical ingredients, including alkaloids, glycosides, carbohydrates, flavonoids, saponins, terpenoids, taninns, and phytosterols, were found in the phytochemical screening results. Bougainvillea glabra leaf powder's fluorescence study revealed distinctive colours with different compounds. Heavy metal concentrations of cobalt, mercury, nickel, silver, and zinc were found to be negative. TLC of alcoholic and aqueous extracts of Bougainvillea glabra showed bands with Rf 0.4–0.6, suggesting coumarin and flavonoids presence; further spectroscopic analysis is required for confirmation. Conclusion: In conclusion, Bougainvillea glabra leaf extracts include bioactive natural compounds that can be utilized in the creation of novel medications that improve therapeutic use.